Absorbance vs cell concentration from spectrophotometry


Hi dear,

I have plotted a graph for absorbance at 540 nm vs cells concentration of yeast for dilutions from 1/2 to 1/128. Using this graph, I can find cell concentration of unknown yeast after measuring its absorbance. It is possible to for bacterial cells as well? Can you determine the concentration of bacterial cell if i know the absorbance? If yes or no, Why?

Thank you very much


Dawt 7 months 0 Answers 299 views New Member 0

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