Absorbance vs cell concentration from spectrophotometry
I have plotted a graph for absorbance at 540 nm vs cells concentration of yeast for dilutions from 1/2 to 1/128. Using this graph, I can find cell concentration of unknown yeast after measuring its absorbance. It is possible to for bacterial cells as well? Can you determine the concentration of bacterial cell if i know the absorbance? If yes or no, Why?
Thank you very much