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Centrifuge machine

Centrifuge machine

It is use for centrifugation

Principle:

Separate molecule on the basis of size and density. If size decrease it required high centrifugation

The method in which we increase gravity by increasing rotation the increase in the centrifugal force

Relative Centrifugal Force/RPM:

Gravity produced in centrifuge is RCF. The speed at which centrifuge machine rotate if ‘g’ value is known we can calculate rpm. If we have rpm we can calculate ‘g’ value by

RCF(g)=11.81x(RPM/1000)2

We can calculate RPM by

RPM= 299.07 √RCF/r

‘r’ it is the radius of rotor

TYPES OF CENTRIFUGE MACHINE:

On the basis of RCF /speed

LOW SPEED CENTRIFUGE:

Rotate till 10,000 rpm

High speed centrifuge:

Revolution till 30,000rpm

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Ultra-Centrifugation:

Higher than 30,000rpm

Image result for ultracentrifuge machine

Parts:

ROTOR:

Bucket in which we place tubes it rotates by motor (fixed angle rotor)

MOTOR

TACHOMETER:

adjust rpm /min and also help in calibration of rpm. Digital tachometer is also available.

IMBALANCE DETECTOR

REFRIGERATION:

provides cooling system because when motor rotate fast it generate heat.

VACUUM PUMP:

when the speed reaches to the 30,000-rpm vacuum pump remove the air to control the speed

LID:

it is important to close the lid otherwise machine stop. In any emergency case (spill or brakeage of tubes lid will b remain close even the machine stops and then there is a hole present due to which we open the lid with the help of key

SCREEN

SWING BUCKET:

when rotor move bucket also move in vernicle position. we can centrifuge

96 well plate also it is use for small pallet. Each rotor has specific capacity. We can use different/multiple rotors in one machine

ADOPTOR:

carry tubes in a rotor

POTENTIOMETER:

control electric current potential. Provide high speed to rotor. It is present in a motor.

CALIBRATION:

temperature calibration is done by thermometer and time by timer

CLEANING:

with brush and water and then lubricate it after washing

STERILIZATION:

70% alcohol is used

DENSITY GRADIENT CENTRIFUGATION:

separation on the basis of density different layers are formed. According to density sample preparation in that specific layers.

Polystyrenes tubes are use

In ultra-centrifugation metal tubes are use

BRAKE:

deaccelerate the speed to stop suddenly, the centrifugation

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