Dna binding proteins

Question

How experimentally would you detemine a protein is dna binding ?

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Sam4545 2020-08-19T21:32:15+00:00 1 Answer 182 views New Member 0

Answer ( 1 )

  1. Microbe-lover
    0
    2020-12-14T07:08:06+00:00
    Reply

    different forces is involves while interacting DNA with the protein i.e. hydrogen bond, dipolar interaction etc. .. you can perform different experiments to find the DNA -protein interaction and the following method is:

    • Down pull down assay
    • micro plate capture and  detection assay
    • DNA electrophoretic mobility shift assay
    • chromatin immuoprecipitation assay

    These experiments helps u to find our your answer..

    also i would prefer you to read this article for further information .. it will definitely help you out

    https://www.thermofisher.com/pk/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/methods-detecting-protein-dna-interactions.html

    hope you got the point

    Thanks

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