how to increase protein expression in e coli ?
Question
how to increase protein expression in e coli
Donald K. Day
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how to increase protein expression in e coli
Answer ( 1 )
select the most suitable adaptive E.coli strain . then select the best expression vector of transfer of gene of interest (protein of which you want to increase expression). do optimization of codons. set the conditions i.e. use low expression temperature. give optimum temperature and media for production of protein.