What microscope features are necessary for studying fungi?

Question

I have been collecting and studying samples of fungi/lichen in my area under a microscope for a while now, (mostly basidiomycetes so far since they’re all I understand, but am looking to expand my knowledge,) and I’m now looking to upgrade since mine is essentially a very nice toy (plastic condenser lens, stage clips instead of mechanical stage, etc). What features should I look for that are generally present on a microscope used for routine mycological work? And does anyone have any other advice I might want to hear related to buying a microscope for mycology?

Currently, I’m looking at getting a chinese-made scope with Köhler illumination and a filter holder for the condenser so that I can do things like darkfield microscopy if need be. (I know that these scopes are a point of contention in the microscopy world, but I don’t think the quality will make a difference for my purposes. This is it: https://tinyurl.com/eyv77wmx) The scope does NOT have infinity optics, so I won’t be able to put in prisms or filters in the tube to do things like DIC microscopy, so if that is important in the mycological world I want to know so I won’t end up burned if I need it.

The mycology books I’m reading often mention needing things like phase contrast microscopy to see certain features, and I feel I don’t have a good sense of what features I actually NEED in a microscope. The goal is simply to have a microscope that I can see all the important features for studying/identifying fungi on a more academic level, like identifying different hyphal incrustastions, spore shapes/textures, or features of basidia/cystidia. I’m willing to put in lots of time and effort into jurry-rigging any potential solutions, too, if it means saving money.

I hope that wasn’t too much rambling, and I hope I made clear what I need. Either way, thanks for your time, and thanks in advance if you give a reply!

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Jonah French-Jurgens 2022-11-02T07:55:48+00:00 2 Answers 14 views New Member 0

Answers ( 2 )

    0
    2022-11-02T10:19:07+00:00

    I hope You are in good health. there are a lot of microscopes depending upon for which purpose you need to. for mycology, you can use a light microscope which you may have used in your previous study.

    if you want further detailing you can use

    • Phase contrast microscope: you can see the image slightly clear without staining
    • fluorescent microscope: you can see images based on the fluorescent stain you add
    • electron microscope: electron microscope is highly efficient in work because we use electrons to make images instead of light sources.
    0
    2022-12-23T18:19:05+00:00
    This answer was edited.

    to study fungal spores like basidia, cystidia you need a microscope of at least 400X; 1000x magnification should ideally be present.
    dissecting microscopes used to study fungal structure.
    scanning electron microscopes are also very good.
    regards

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